Rapid advancements in the genomics and molecular biology especially various genome sequencing projects, created a new major area of interest to the biologists, Bioinformatics, where biology merges with the computer science and information technology. Bioinformatics continue to be the main focus of attraction in the coming years, especially in the livestock production, as it will find more application in diagnostics, vaccine development, veterinary medicine, animal breeding and various disciplines of animal sciences. Also the advancements in next generation sequencing technologies will assist the animal scientists to understand the molecular and cellular mechanisms to different environmental conditions. This book acquaints the readers with the basic bioinformatic tools which are essential for any molecular biology work like Various databases, Primer designing, Sequence alignment, Submission to Bankit and Molecular Phylogeny analysis. Further the book also introduces and details various advanced topics like Real time PCR, Next Generation Sequencing, Transcriptome Analysis, Genome Annotation, Computational Approach for development of DNA signature and applications of marker based technologies in Animal Breeding. The simplicity of language and clear illustrations in various bioinformatics soft-wares will help even a beginner to learn the tools with a clear understanding. The concise and clearly written book will provide invaluable resources for both the beginners and established researchers in various fields of animal sciences; as a well acquaintance with basic and advanced bioinformatic and in-silico tools can be acquired through the book.